Propolis HPLC Analysis

Propolis obtained from honeybee hives has been widely used in medicine, cosmetics, and industry due to its versatile biological activities (antioxidant, antimicrobial, fungicidal, antiviral, antiulcer, immunostimulating, and cytostatic). These activities are mainly attributed to the presence of flavonoids in propolis, which points out the interest in quantifying these constituents in propolis preparations, as well as validation of analytical methodologies. High-performance liquid chromatography (HPLC) methods have been reported to quantify isolated flavonoids or these compounds in complex biological matrices, such as herbal raw materials and extractive preparations. An efficient, precise, and reliable method was developed for quantification of propolis extractive solution using HPLC with UV detection. The chromatograms were obtained from various gradient elution systems (GES) tested in order to establish the ideal conditions for the analysis of propolis extractive solution, using methanol and water:acetonitrile (97.5:2.5, v/v) as mobile phase. Gradient reversed phase chromatography was performed using a stainless steel column (250 times 4.6 mm i.d., 5 µm) filled with Chromsep RP 18 (Varian), column temperature at 30.0 ± 0.1°C and detection at 310 nm. The main validation parameters of the method were also determined. The method showed linearity for chrysin in the range 0.24-2.4 µg mL-1 with good correlation coefficients (0.9975). Precision and accuracy were determined. The obtained results demonstrate the efficiency of the proposed method. The analytical procedure is reliable and offers advantages in terms of speed and cost of reagents.

Melia Propolis

Q.S. AN NAHL : Ayat. 68 & 69
……..Keluarlah dari perutnya syaraabun (cairan) beraneka warna, dan padanya syifa (penyembuhan) bagi manusia. Sesungguhnya pada yang demikian itu menjadi tanda bagi kaum yang memikirkan.

Kandungan Propolis:
1. Bioflavonoids: Memulihkan system kapilari serta memperbaiki kerapuhan dan kebocoran saluran darah (1 tetes propolis kandungannya setara dengan 500 buah jeruk)
2. Protein ( 16 Asam amino Esensial)
3. Vitamin dan mineral

Fungsi Propolis bagi manusia
1. Detoksifikasi (Membuang racun dan Kuman Penyakit dari dalam tubuh)
2. Antibiotika Alami (antimicrobial seperti virus, bakteri dan jamur)
3. Anti Radang
4. Anti Alergi
5. Meningkatkan Imunitas / Kekebalan Tubuh
6. Anti Oksidan (mencegah kanker dan membunuh sel kanker)
7. Nutrisi (memperbaiki dan regenerasi sel tubuh)